This paleolimnological study, based on the analysis of fossilized diatoms and sedimentological properties, presents the stratigraphic profile of a sediment core taken from lac Papineau (Ottawa River valley) in spring 2018. The lake is located in a region with a rich anthropogenic history, and the discovery of a pirogue in autumn 2017 attracted high interest for archaeological and paleolimnological studies. The core has been analyzed for organic matter content, particle-size grading as well as the changes in the abundance and diversity of diatoms. The overall goal of this study is to provide the limnological and climatological context for the time when the pirogue was used by humans in the area.